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Image Search Results
Journal: STAR Protocols
Article Title: Protocol for quantifying drug sensitivity in 3D patient-derived ovarian cancer models
doi: 10.1016/j.xpro.2024.103274
Figure Lengend Snippet:
Article Snippet:
Techniques: Immunofluorescence, Flow Cytometry, Control, Recombinant, Red Blood Cell Lysis, Saline, Software, Cell Culture, Plasmid Preparation, Microscopy
Journal: PLoS Pathogens
Article Title: Receptor-Targeted Nipah Virus Glycoproteins Improve Cell-Type Selective Gene Delivery and Reveal a Preference for Membrane-Proximal Cell Attachment
doi: 10.1371/journal.ppat.1005641
Figure Lengend Snippet: ( A ) Cell entry is EpCAM dependent but independent of ephrin-B2. Representative flow cytometry plots out of three independent experiments of CHO-K1, CHO-EpCAM, CHO-ephrin-B2 cells, and of a mixed culture composed of CHO-EpCAM and CHO-ephrin-B2 (1:1 ratio) monitored 72 h after transduction with NiVmut EpCAM -LV, NiVwt-LV or VSV-LV (MOI of 1). EpCAM expression was detected by an APC-coupled human EpCAM specific antibody. ( B ) To ascertain stability of transduction with the EpCAM-targeted vector, CHO-EpCAM cells were cultivated for further 30 days after transduction with the indicated MOIs. The percentage of GFP-positive cells was determined by flow cytometry at the indicated time points. One representative out of three independent experiments is shown.
Article Snippet: Human EpCAM was detected by an Allophycocyanin (APC) labeled
Techniques: Flow Cytometry, Transduction, Expressing, Plasmid Preparation